Journal: International Journal of Molecular Sciences

Article Title: Oral Sodium Hyaluronate Reshapes Gut Microbiota Composition and Suppresses the LPS-TLR4/NF-κB Pathway to Exert Neuroprotection in MPTP-Induced Parkinson’s Disease

doi: 10.3390/ijms27156573

Figure Lengend Snippet: Oral SH inhibited the TLR4/MyD88/NF-κB signaling pathways both in the SN and the colon of MPTP-induced mice. ( A ) Schematic representation of the signaling pathway. ( B ) Representative WB bands of TLR4, MyD88, and NF-κB in the colon. ( C ) Representative WB bands of TLR4, MyD88, and NF-κB in the midbrain containing the SN. ( D – F ) The density analysis results of TLR4, MyD88, and NF-κB in the colon. ( G – I ) The density analysis results of TLR4, MyD88, and NF-κB in the midbrain containing the SN. ( n = 3 for each group. Data are presented as mean ± SD. *** p < 0.001 versus the control group; ### p < 0.001 versus the MPTP group.).

Article Snippet: Membranes were incubated at 4 °C overnight with the following primary antibodies: rabbit anti-β-actin antibody (1:50,000, AC026, Abclonal, Wuhan, China), mouse anti-TH antibody (1:1000, A0028, Abclonal, Wuhan, China), rabbit anti-TNF-α antibody (1:1000, A20851, Abclonal, Wuhan, China), rabbit anti-IL-1β antibody (1:1000, A11369, Abclonal, Wuhan, China), rabbit anti-IL-6 antibody (1:1000, A0286, Abclonal, Wuhan, China), goat anti-COX-2 antibody (1:1000, A1253, Abclonal, Wuhan, China), mouse anti-TLR4 antibody (1:1000, A5258, Abclonal, Wuhan, China), rabbit anti-MyD88 antibody (1:400, A0980, Abclonal, Wuhan, China), rabbit anti-IκB-α antibody (1:1000, A24742, Abclonal, Wuhan, China), rabbit anti-ZO-1 antibody (1:1000, A0659, Abclonal, Wuhan, China), mouse anti-claudin-1 antibody (1:1000, Abclonal, Wuhan, China), rabbit anti-occludin antibody (1:1000, Abclonal, China), and mouse anti-claudin-5 antibody (1:1000, A10207 , Abclonal, Wuhan, China).

Techniques: Protein-Protein interactions, Control